Studies on Hexokinase. Part II

Hexokinase II of Pea Seeds.

A second hexokinase (EC 2.7.1.1) was obtained from pea seed (Pisum sativum L. var. Progress No. 9) extracts. The enzyme, termed hexokinase II, had a high affinity (K(m), 48 micromolar) for glucose and a relatively low affinity (K(m), 10 millimolar) for fructose. The K(m) for MgATP was 86 micromolar. Mg(2+) was required for activity, but excess Mg(2+) was inhibitory. MgADP inhibited hexokinase I...

Kinetic studies of yeast hexokinase.

The mechanism of calf brain hexokinase has recently been studied in our laboratory (1). It was concluded from kinetic experiments that adenosine diphosphate (ADP) dissociates from the enzyme before addition of substrate glucose. This hypothesis was supported by kinetic studies of n-mannose, ADP, and glucose g-phosphate inhibition. The experiments appear to lend credence to theories in which an ...

Studies on the Standardisation of Curnas Part – Ii Talizadya Curna

Talisadya churna was prepared by pounding the individual ingredients in mortar and pestle and mixie. The Curna prepared by pounding the ingredients in mortar and pestle showed higher exhaustive extraction in hexane and solubility in alcohol. The Curna prepared by grinding the ingredients in mixie showed less acid insoluble content, high volatile matter, water soluble matter, and exhaustive extr...

Part II Laminar Flame Dynamics Studies

Mammography; Part II

From the Book of Mammography by Dr. Zabihollah Arnavaz - Head of Radiology Department of Jorjani Hospital of National University of Iran.